Shameik Moore Miles Morales / Spider-Man (voice) Jake Johnson Peter B. Parker / Spider-Man (voice) Hailee Steinfeld Gwen Stacy / Spider-Woman (voice) Mahershala Ali Uncle Aaron / Prowler (voice) Brian Tyree Henry Jefferson Davis (voice) Lily Tomlin Aunt May (voice) Luna Lauren Velez Rio Morales (voice) Zoë Kravitz Mary Jane (voice) John Mulaney Peter Porker / Spider-Ham (voice) Kimiko Glenn Peni Parker (voice) Nicolas Cage Peter Parker / Spider-Man Noir (voice) Kathryn Hahn Olivia 'Liv' Octavius / Doc Ock (voice) Liev Schreiber Wilson Fisk / Kingpin (voice) Chris Pine Peter Parker / Spider-Man (voice) Natalie Morales Miss Calleros (voice) Edwin H. Bravo Brooklyn Visions Security Guard (voice) Oscar Isaac Interesting Person #1 (voice) Greta Lee Interesting Person #2 (voice) Stan Lee Stan (voice) Jorma Taccone Green Goblin / Last Dude (voice) Joaquín Cosío Scorpion (voice) Marvin 'Krondon' Jones III Tombstone (voice) Kim Yarbrough Scientist in Cafeteria (voice) Lake Bell Vanessa Fisk (voice) Jessica Mikayla Adams Brooklyn Friend (voice) Sarah D. Cole Brooklyn Friend (voice) Mimi Davila Brooklyn Visions Student (voice) Claudia Choi Brooklyn Visions Teacher (voice) Post Malone Brooklyn Bystander (voice) Scott Menville Additional Voices (voice) Al Rodrigo Additional Voices (voice)